PCR uses primers to copy only the segment of DNA you’re looking for. Primers contain the complements of the first few “letters” of the “word” you’re looking for on your template strand (the first bases of your target sequence). They only bind to the template strand where they find those letters, and thus only those sequences are copied. Real-time PCR works by introducing fluorescent dyes and probes to the PCR process. Devices can then optically measure how much fluorescence there is in your reaction mix and use that to interpret how much target DNA was in your sample.
Real-time PCR is an updated PCR method that allows you to receive results as the reaction proceeds. Instead of waiting for the entire chain reaction to finish, you can watch the reaction proceed in “real time”.
Our device takes approximately 30 – 55 minutes for a complete run. Contact us for the latest spec sheets.
No, all of our products are currently for Research Use Only and not for use in diagnostic procedures. We intend to take a subset of our products through the US regulatory process in the near future.
Currently our device is compatible with FAM/SYBR and Cy5/ATTO647N fluorophores. Isothermal (LAMP) analysis is also possible. Contact us for the latest device spec sheets.
We have developed several master mixes: LyoDNA, LyoRNA, and LyoGreen. We also offer Go-Strips with lyophilized master mix. These products are available for sale at our store.
You can also use your favorite commercial or homebrewed master mixes. Note that your master mix should not contain ROX.
We have a number of test kits available for sale at our store. You also have the option of developing your own test using our bulk product line. Other organizations are also developing their own tests for use on the Biomeme platform in a variety of industries and applications ranging from biothreat to nutrigenomics to K-12 STEM education. Contact us to discuss potential test development partnerships.
Yes. The Biomeme two3 can run for about 6 test runs on a single charge.
The device comes with a power adapter that plugs directly into a wall outlet. Charging takes about 1.5 hours, and the device will still work while charging. Watch this short video here about how to charge your two3 thermocycler.
On a full charge, the battery can deliver up to six tests.
No! Our device comes with the iPhone SE and all necessary software to get started right away.
Yes, the device is compatible with standard 200 µL PCR tubes. However, we highly recommend using Biomeme’s Go-Strips, as they are specially designed for use with our device.
Yes. Please make sure the device is turned off, and that the smartphone is turned off or in airplane mode before flying.
The device analyzes test results in real time. The data are stored on the iPhone and synced with Biomeme’s cloud-based data management system when the iPhone has a Wifi or cellular connection.
Biomeme’s web portal and accompanying dashboard enables users to track and manage test results seamlessly from anywhere on any device. Users can also manage and view test results within the iOS application and export raw data as a .csv file via email or text. You can also export results as a .jpg file of the amplification plot with a text description.
Results can be securely sent to third-party databases on a case-by-case basis.
We use 128-bit encryption on all data transfer. Human health test data can be stored in a HIPAA-compliant database.
Our data management system is cloud-based and easily integrated with other cloud-based systems. We work with customers to identify an EHR of choice and then go to that EHR to request an API for that customer’s case.
Yes, we have an API that will allow you or your organization to create apps to fit your data management needs.