Currently, our two3 device is compatible with FAM/SYBR and ATTO647N fluorophores. Our three9 device adds a third amber channel between the green and red channels that detects TexasRedX.
Isothermal (LAMP, NASBA, etc.) analysis is also possible. Contact us for the latest device spec sheets.
We have developed several master mixes: LyoDNA, LyoRNA, and LyoGreen. We also offer Go-Strips with lyophilized master mix. These products are available for sale at our store. You can also use your favorite commercial or homebrewed master mixes. Note that your master mix should not contain ROX.
We have a number of test kits available for sale at our store. You also have the option of developing your own test using our bulk product line. Other organizations are also developing their own tests for use on the Biomeme platform in a variety of industries and applications ranging from biothreat to nutrigenomics to K-12 STEM education. Contact us to discuss potential test development partnerships.
The Biomeme two3 technology allows for DNA collection and direct processing in the field, which minimizes DNA degradation that usually occurs if the sample needs to be transported from the field back to a lab (remote from the field) for processing. The two3 allows for sample processing in-the-field so transportation of the sample DNA is not necessary.
There is not a high chance of contamination because, unlike lab protocols, the Biomeme platform makes use of disposable, single-use consumables. Plus, the two3 is easy-to-use with simple step-by-step instructions. Biomeme has been used in the field by numerous researchers without contamination. If desired, a user can always run negative controls at the beginning and/or end of the testing day.
Central lab-centric processing is a completely different model than Biomeme. A central lab-bound process will wait to receive a sufficient quantity of samples and then run all of them in a batch process. In contrast, the Biomeme technology is field-based, end-to-end solution for real-time detection and data analysis for mission-critical, actionable use cases where time is of the essence.
Biomeme’s mobile technology has been used for a wide variety of aquatic eDNA and water quality applications. Biomeme’s M1 sample prep works with up to 1mL of fresh, marine, or industrial process water. The system can also test water filters commonly used in eDNA field collection applications. Read more in a recently published article on eDNA field detection using Smith-Root’s ANDe™ water filtration system and Biomeme M1 sample prep, Go-Strip tests, and two3 hand-held thermocyclers.
The Biomeme sample prep protocol requires NO INCUBATION and the process takes just a few minutes to extract and purify high quality nucleic acids from a variety of sample types.
All of Biomeme’s end-to-end Go-Kits come with everything needed isolated nucleic acids (DNA/RNA) in the field. No lab equipment, no cold-chain, no power, no problem! All the sample prep buffers come pre-aliquoted, and the Go-Strip tests include master mix, primers and probes lyophilized together in shelf-stable PCR strips. These Go-Strips are then put into Biomeme’s battery-powered, hand-held qPCR thermocyclers.
Professional molecular biologists can take advantage of Biomeme’s open architecture to design whatever protocol they desire. Program in your specific thermocycling protocol with a few taps in the mobile app, label the reactions as you like and run as many replicates or positive/negative controls you desire, and after the test is completed you’ll have access to all of your raw data both in the mobile and in the Biomeme web portal. Biomeme’s prepared end-to-end Go-Kits and Go-Strip tests have different layouts depending on the intended use. Most of our eDNA tests are multiplexed with an internal positive control (IPC) to check for inhibition and confirm the device, reagents, and PCR worked correctly. Negative controls are generally less of an issue given that all sample prep and test reagents & plastics are single-use, reducing the risk of contamination. Nevertheless, in some cases users may want to run negatives controls at the beginning and/or end of a test day.
Biomeme considers formulation of our primers as trade secrets: we are happy to share the gene targets. Also, Biomeme offers custom-test manufacturing services if users would like field kits developed with their own primer sequences. Contact us to discuss partnerships. How can we help you do what you do?
ROX Reference Dye is a proprietary Dye designed for use on Thermofisher lab-bound thermocyclers to normalize the fluorescent reporter signal in their real-time qPCR or RT-PCR. Biomeme and numerous trusted, name-brand equipment manufacturers — e.g. Roche and Bio-Rad — do not require the use of a calibrator dyes to normalize their data. This is one additional benefit of using the Biomeme two3.