PCR uses primers to copy only the segment of DNA you’re looking for. Primers contain the complements of the first few “letters” of the “word” you’re looking for on your template strand (the first bases of your target sequence). They only bind to the template strand where they find those letters, and thus only those sequences are copied. Real-time PCR works by introducing fluorescent dyes and probes to the PCR process. Devices can then optically measure how much fluorescence there is in your reaction mix and use that to interpret how much target DNA was in your sample.
Real-time PCR is an updated PCR method that allows you to receive results as the reaction proceeds. Instead of waiting for the entire chain reaction to finish, you can watch the reaction proceed in “real time”.
Our device takes approximately 30-50 minutes for a complete PCR run, but it really depends on the chemistry and specific test protocol. Contact us for the latest spec sheets.
No, all of our products are currently for Research Use Only and not for use in diagnostic procedures. We intend to take a subset of our products through the US regulatory process in the near future. Contact Us if you have a specific application in mind and would like to collaborate.
The Biomeme platform is currently being used for eDNA analysis by many industry leaders, including: Wildlife Conservation Society, Columbus Zoo & The Wilds, Yosemite National Park, Norwegian Veterinary Institute, Cornell University, Smith-Root, Washington State University, University of Guelph Biodiversity Institute, Precision Biomonitoring, and more. Many have published several recent case studies validating the value of the tool for eDNA. Contact Us to get copies of the latest research and/or download our Guide to Environmental DNA (eDNA).
The two3 and three9 are designed for real-time PCR at the point of need: they are designed for applications that are time-sensitive and location-sensitive, where results from the field need to be actionable as soon as possible. Bring the lab to the sample! Factors to consider when choosing the appropriate PCR:
Biomeme is not trying to compete with high-volume throughput use cases in a centralized lab. Instead, Biomeme offers these benefits:
Our use cases are usually time-sensitive and/or location sensitive situations where the real-time result is actionable.
In place of using the two3, higher throughput can be achieved with the new Biomeme thermocycler, the three9: 9 samples (test each sample for 2 targets + 1 IPC).
Yes, the nucleic acid in prepared samples is relatively stable and can be brought back to the lab for further testing.
The two3 and three9 thermocyclers can do 5-6 runs before having to recharge (recharging takes about 1.5 hours and they can be plugged in and charge while also running). A 40-cycle PCR run takes about 45min on either device. If you run 1 sample per PCR well then that means you can test 3 or 9 samples in under an hour for the two3 or three9, respectively. You could start by preparing some samples with our M1 Sample Prep, then get the PCR run started and start preparing the next set of samples. In this way, a single person with one device could prepare and PCR approximately 18 samples with the two3 or 54 samples with the three9 in a typical day.