Bundibugyo virus (BDBV)
Lyophilized, shelf-stable nucleic acid amplification test for rapid detection of Bundibugyo ebolavirus RNA. Available in pre-aliquoted 96-well plates or bulk reaction vials. No extraction required.
What's in the Kit
Bundibugyo virus 96-well Go-Plate or Go-Vial
Lyophilized duplex reactions with master mix, enzymes, guides, and probes for all targets.
SampleDirect™ Lysis Buffer
Bulk bottle. Liberates nucleic acids from the sample matrix and inactivates degrading nucleases.
SampleDirect™ Recovery Buffer
Bulk bottle. Renders the lysate compatible with nucleic acid amplification chemistry.
Go-Plates™ — Flexible by Design
$1,249 · 96 rxnsGo-Plates are pre-aliquoted, lyophilized plates for lab use in standard commercial thermocyclers. Run the full 96-well plate or cut away and run as strips in any direction.
Each well contains a 20 µL lyophilized duplex reaction with master mix, guides, and probes for all targets. A multiplexed reaction means both targets are in a single reaction — one well equals one sample or replicate.
Compatible with almost all PCR and qPCR cycler models which accept Regular Profile (RP) products, such as ABI/Life Technologies, Agilent, Bio-Rad, Eppendorf, and others.
Cuttable — Multiples of 8
Cuttable — Multiples of 12
Go-Vials™ — Ultimate Flexibility
$649 · 600 µL mixGo-Vials are lyophilized for lab use in standard commercial thermocyclers and your own plastics of choice. Run the full 600 µL reaction volume or freeze for later use.
Each vial contains a 600 µL lyophilized duplex reaction with master mix, guides, and probes for all targets. A multiplexed reaction means both targets are in a single reaction — one well equals one sample or replicate.
Compatible with all PCR and qPCR cycler models which detect Cy5 and Cy5.5 fluorophores.
Reaction Yield by Reaction Volume (600 µL Bulk)
reactions
Maximum yield for micro-volume protocols
reactions
Optimized for high-throughput screening
reactions
Standard reaction volume equivalent to Go-Plate
reactions
Higher template volume for maximum sensitivity
Aliquot into your own tubes or plates at the reaction volume required by your protocol.
Assay Specifications
| Product Name | Bundibugyo virus Go-Plate Kit / Go-Vial Kit |
| Price | $1,249 (Go-Plate) / $649 (Go-Vial) |
| Extraction Required | No |
| Specimen Type | EDTA Whole Blood (volume flexible) |
| Reaction Volume | 20 µL (Go-Plate) / Flexible (Go-Vial) |
| Time-to-Result | ~10 minutes |
| Storage Temperature | 15–30°C (room temperature) |
| Color Channels | Cy5, Cy5.5 |
| Target 1 | Bundibugyo virus RNA (BDBV) |
| Target 2 | Endogenous Positive Control (PPIB) |
| Form Factor | 96 × 0.1 mL plate (cuttable into strips) / Go-Vial |
| Compatible Cyclers | Bio-Rad CFX96, ABI QuantStudio 5+, Agilent, Eppendorf, and other standard RP-profile cyclers / Any cycler able to detect Cy5 and Cy5.5 |
Protocol
From raw sample to result in approximately 10 minutes. No extraction, no purification, no complex master mix prep.
Lyse
Add EDTA whole blood into SampleDirect™ lysis buffer.
Recover
Add SampleDirect™ recovery buffer into the lysate.
Transfer
Transfer lysed and recovered sample mixture to the assay reaction well.
Amplify
Heat at 58°C for 10 minutes. Read results.
About Bundibugyo virus
Bundibugyo virus (BDBV) is a species of ebolavirus closely related to the Zaire ebolavirus (EBOV). It is one of several species that can cause Ebola virus disease (EVD) in humans, manifesting as viral hemorrhagic fever with high mortality rates.
First identified during a 2007 outbreak in the Bundibugyo District of Uganda, BDBV presents a significant biosecurity and public health concern. Rapid, accurate identification of the specific ebolavirus species is critical for outbreak response, contact tracing, and clinical management — particularly in resource-limited and austere field environments where central laboratory infrastructure may be unavailable.
Available in Go-Plate and Go-Vial formats, this assay enables detection of BDBV RNA directly from EDTA whole blood in approximately 10 minutes, with no nucleic acid extraction step required. An endogenous human RNA control (PPIB) is included to verify sample integrity.
Biosafety Classification
Bundibugyo virus is classified as a Risk Group 4 pathogen. Handle specimens in accordance with applicable biosafety regulations and institutional guidelines.
Assay Targets
BDBV
Bundibugyo ebolavirus genomic RNA
PPIB
Endogenous human RNA positive control
About the Chemistry
Biomeme's proprietary isothermal chemistry system was developed out of learnings from the COVID pandemic — when supply chains collapsed, labs faced scaling challenges, and labor shortages crippled testing capacity.
SampleDirect™
Sample Preparation
A 2-component system that prepares nucleic acids for amplification. No purification or additional buffer systems needed.
Step 1: Crude sample is added to the lysis buffer, which liberates nucleic acids from the matrix and inactivates degrading nucleases.
Step 2: The lysed sample is mixed with the recovery buffer, which renders the lysate compatible with nucleic acid amplification chemistry.
Tested Matrices
Target Flexibility
DTECT™
Amplification Chemistry
Differential Targeted Endonuclease Cleavage Technology — a strand-displacement method employing a novel guide molecule that introduces an exogenous endonuclease restriction site to the target locus of interest.
The System: 2 enzymes (a polymerase and a site-specific nicking endonuclease) + 2 guide oligonucleotides, paired with a Molecular Beacon probe for detection and multiplexing. RNA amplification requires an additional reverse transcriptase and primer.
The DTECT chemistry is lyophilized — pre-mixed, pre-aliquoted, and shelf-stable — eliminating the need for cold-chain logistics and manual reagent preparation.
Reaction Components
Key Advantages
No Cold Chain
Ships and stores at room temperature (15–30°C). No dry ice shipment, no −80°C freezer. Eliminates the risk of degradation from freeze-thaw cycles.
No Mixing (or Aliquoting for Go-Plates)
Each Go-Plate well is pre-mixed, pre-aliquoted, and lyophilized. For Go-Vials, reconstitute the bulk mix and aliquot into your preferred volumes. No molecular biologist required. No complex master mix prep.
Maximum Sensitivity
Because each test is lyophilized, you can add more sample template (20 µL in Go-Plates, or flexible volumes in Go-Vials) to directly increase analytical sensitivity without altering reagent concentrations.
Standard Equipment
Runs on standard cyclers: Go-Plates fit regular profile (RP) cyclers (Bio-Rad, ABI, Agilent, Eppendorf), while Go-Vials work with your own plastics on any cycler detecting Cy5 and Cy5.5.
10-Minute Results
From raw sample to actionable result in approximately 10 minutes. Lightning-fast isothermal amplification with no compromise on sensitivity.
Endogenous Control
Includes a human RNA positive control (PPIB) in every reaction to verify sample integrity and confirm successful nucleic acid recovery.
Order Bundibugyo virus Go-Plate or Go-Vial Kits
Ready to deploy rapid BDBV detection in your laboratory? Contact our sales team to place an order or request additional information.
$1,249 (Go-Plate, 96 rxns) · $649 (Go-Vial, 600 µL mix) · No extraction required · Ships at room temperature
For Research Use Only. Not for use in human or veterinary diagnostics. The performance characteristics of this product have not been established.